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to research the constraints imposed by Lon about the DHFR mutational landscape in structural element, we outlined a ∆range coefficient for each amino acid residue at Every place as the difference between the +Lon and –Lon alternatives (Figure 3A). The ∆range coefficient values ended up most unfavorable at positions inside the Beneficial category and at positions with a native VILMWF or Y amino acid residue (Figure 3B, excludes Intolerant positions from –Lon variety); All round, mutations at positions with native hydrophobic residues are enriched for destructive ∆selection coefficients (Figure three—figure health supplement 1A).
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